Coding

Part:BBa_K785003:Design

Designed by: Zining Hou   Group: iGEM12_Fudan_Lux   (2012-09-23)


Lux(CDABEG) coding gene PoPS->Light


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1959
    Illegal XhoI site found at 2789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4348
    Illegal BsaI.rc site found at 1357
    Illegal SapI.rc site found at 4673


Design Notes

In our project, we have to put the Lux light generator gene under the Ptet operon. So we have to relieve this part from the Pbad operon.


Source

We use PCR extract the DNA sequence from BBa_K325909, and clone it into the PSB1C3.

References